Recombinant Escherichia coli Ornithine carbamoyltransferase subunit F (argF) | CSB-RP129074Ba

Recombinant Escherichia coli Ornithine carbamoyltransferase subunit F (argF) | CSB-RP129074Ba | Cusabio

Alternative Name(s): OTCase-2

Gene Names: argF

Research Areas: Others

Organism: Escherichia coli (strain K12)

AA Sequence: SDLYKKHFLKLLDFTPAQFTSLLTLAAQLKADKKNGKEVQKLTGKNIALIFEKDSTRTRCSFEVAAFDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYDGIQYRGHGQEVVETLAQYAGVPVWNGLTNEFHPTQLLADLMTMQEHLPGKAFNEMTLVYAGDARNNMGNSMLEAAALTGLDLRLLAPKACWPEESLVAECSALAEKHGGKITLTEDVAAGVKGADFIYTDVWVSMGEAKEKWAERIALLRGYQVNAQMMALTDNPNVKFLHCLPAFHDDQTTLGKQMAKEFDLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMMATLGE

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 2-334aa

Sequence Info: Full Length of Mature Protein

MW: 40.7 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.

Reference: Evolutionary divergence of genes for ornithine and aspartate carbamoyl-transferases -- complete sequence and mode of regulation of the Escherichia coli argF gene; comparison of argF with argI and pyrB.van Vliet F., Cunin R., Jacobs A., Piette J., Gigot D., Lauwereys M., Pierard A., Glansdorff N.Nucleic Acids Res. 12:6277-6289(1984)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.

Involvement in disease:

Subcellular Location: Cytoplasm

Protein Families: ATCase/OTCase family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P06960

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A