Recombinant Escherichia coli O157:H7 Stringent starvation protein B (sspB) | CSB-EP360347EOD
- 13 - 23 Working Days
Recombinant Escherichia coli O157:H7 Stringent starvation protein B (sspB) | CSB-EP360347EOD | Cusabio
Alternative Name(s): sspB; Z4586; ECs4101Stringent starvation protein B
Gene Names: sspB
Research Areas: Others
Organism: Escherichia coli O157:H7
AA Sequence: MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPMEYARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGTMFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPALRVVK
Tag Info: N-terminal 6xHis-tagged
Expression Region: 1-165aa
Sequence Info: Full Length
MW: 22.3 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth. The exact function of SspB is not yet known
Reference: "Dual regulatory pathways of flagellar gene expression by ClpXP protease in enterohaemorrhagic Escherichia coli." Kitagawa R., Takaya A., Yamamoto T. Microbiology (Reading, Engl.) 157:3094-3103(2011)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth. The exact function of SspB is not yet known (By similarity).
Involvement in disease:
Protein Families: SspB family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P0AFZ4
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A