Recombinant Escherichia coli Flagellin (fliC) | CSB-EP300968ENVe1

Recombinant Escherichia coli Flagellin (fliC) | CSB-EP300968ENVe1 | Cusabio

Alternative Name(s): fliC; flaF; hag; b1923; JW1908Flagellin

Gene Names: fliC

Research Areas: Immunology

Organism: Escherichia coli (strain K12)

AA Sequence: AQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRVRELTVQATTGTNSESDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKNGSMKIQVGANDNQTITIDLKQIDAKTLGLDGFSVKNNDTVTTSAPVTAFGATTTNNIKLTGITLSTEAATDTGGTNPASIEGVYTDNGNDYYAKITGGDNDGKYYAVTVANDGTVTMATGATANATVTDANTTKATTITSGGTPVQIDNTAGSATANLGAVSLVKLQDSKGNDTDTYALKDTNGNLYAADVNETTGAVSVKTITYTDSSGAASSPTAVKLGGDDGKTEVVDIDGKTYDSADLNGGNLQTGLTAGGEALTAVANGKTTDPLKALDDAIASVDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG

Source: E.coli

Tag Info: Tag-Free

Expression Region: 2-498aa

Sequence Info: Full Length of Mature Protein

MW: 51.2 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.

Reference: "Benchmarking yeast two-hybrid systems using the interactions of bacterial motility proteins." Rajagopala S.V., Hughes K.T., Uetz P. Proteomics 9:5296-5302(2009)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.

Involvement in disease:

Subcellular Location: Secreted, Bacterial flagellum

Protein Families: Bacterial flagellin family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P04949

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A