Recombinant Escherichia coli Fe/S biogenesis protein NfuA (nfuA), Biotinylated | CSB-EP540344ENT-B

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  • Recombinant Escherichia coli Fe/S biogenesis protein NfuA (nfuA), Biotinylated
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
€482.00 - €1,832.00


Recombinant Escherichia coli Fe/S biogenesis protein NfuA (nfuA), Biotinylated | CSB-EP540344ENT-B | Cusabio

Alternative Name(s):

Gene Names: nfuA

Research Areas: Others

Organism: ENT-Escherichia coli (strain K12 / DH10B)


Source: E.coli

Tag Info: N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged

Expression Region: 1-191aa

Sequence Info: Full Length

MW: 68.8 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Involved in iron-sulfur cluster biogenesis. Binds a 4Fe-4S cluster, can transfer this cluster to apoproteins, and thereby intervenes in the maturation of Fe/S proteins. Could also act as a scaffold/chaperone for damaged Fe/S proteins.

Reference: "The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse." Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R. J. Bacteriol. 190:2597-2606(2008)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.


Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:


Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: B1X760

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A

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