Cusabio Escherichia coli Recombinants
Recombinant Escherichia coli Cytolethal distending toxin subunit A (cdtA) | CSB-EP672721ENL
- SKU:
- CSB-EP672721ENL
- Availability:
- 13 - 23 Working Days
Description
Recombinant Escherichia coli Cytolethal distending toxin subunit A (cdtA) | CSB-EP672721ENL | Cusabio
Alternative Name(s): Cytolethal distending toxin subunit A(CDT A)
Gene Names: cdtA
Research Areas: Others
Organism: Escherichia coli
AA Sequence: CSSGKNKAYLDPKVFPPQVEGGPTVPSPDEPGLPLPGPGPALPTNGAIPIPEPGTAPAVSLMNMDGSVLTMWSRGAGSSLWAYYIGDSNSFGELRNWQIMPGTRPNTIQFRNVDVGTCMTSFPGFKGGVQLSTAPCKFGPERFDFQPMATRNGNYQLKSLSTGLCIRANFLGRTPSSPYATTLTMERCPSSGEKNFEFMWSISEPLRPALATIAKPEIRPFPPQPIEPDEHSTGGEQ
Source: E.coli
Tag Info: N-terminal 6xHis-tagged
Expression Region: 22-258aa
Sequence Info: Full Length of Mature Protein
MW: 29.5 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: CDTs are cytotoxins which induce host cell distension, growth arrest in G2/M phase, nucleus swelling, and chromatin fragmentation in HeLa cells. CdtA, along with CdtC, probably forms a heterodimeric subunit required for the delivery of CdtB.
Reference: "Cloning, sequencing, and expression of the Escherichia coli cytolethal distending toxin genes." Pickett C.L., Cottle D.L., Pesci E.C., Bikah G. Infect. Immun. 62:1046-1051(1994)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function:
Involvement in disease:
Subcellular Location:
Protein Families:
Tissue Specificity:
Paythway:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: Q46668
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A