Recombinant Entamoeba histolytica Calreticulin | CSB-EP004458EKM
- 13 - 23 Working Days
Recombinant Entamoeba histolytica Calreticulin | CSB-EP004458EKM | Cusabio
Alternative Name(s): Calreticulin; Fragment
Gene Names: N/A
Research Areas: Tags & Cell Markers
Organism: Entamoeba histolytica
AA Sequence: KVYFEETFENGWKRIWSKW
Tag Info: N-terminal GST-tagged
Expression Region: 1-19aa
Sequence Info: Full Length
MW: 29.5 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER
Reference: "Calreticulin-like molecule in trophozoites of Entamoeba histolytica HM1:IMSS (SwissProt: accession P83003)."Gonzalez E., Rico G., Mendoza G., Ramos F., Garcia G., Moran P., Valadez A., Melendro E.I., Ximenez C.Am. J. Trop. Med. Hyg. 67:636-639(2002)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (By similarity).
Involvement in disease:
Subcellular Location: Endoplasmic reticulum lumen
Protein Families: Calreticulin family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P83003
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A