Recombinant Entamoeba histolytica Calreticulin | CSB-EP004458EKM

Recombinant Entamoeba histolytica Calreticulin | CSB-EP004458EKM | Cusabio

Alternative Name(s): Calreticulin; Fragment

Gene Names: N/A

Research Areas: Tags & Cell Markers

Organism: Entamoeba histolytica

AA Sequence: KVYFEETFENGWKRIWSKW

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 1-19aa

Sequence Info: Full Length

MW: 29.5 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER

Reference: "Calreticulin-like molecule in trophozoites of Entamoeba histolytica HM1:IMSS (SwissProt: accession P83003)."Gonzalez E., Rico G., Mendoza G., Ramos F., Garcia G., Moran P., Valadez A., Melendro E.I., Ximenez C.Am. J. Trop. Med. Hyg. 67:636-639(2002)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (By similarity).

Involvement in disease:

Subcellular Location: Endoplasmic reticulum lumen

Protein Families: Calreticulin family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P83003

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A