Recombinant Eisenia foetida Lysenin-related protein 2 | CSB-EP517722EJV

Recombinant Eisenia foetida Lysenin-related protein 2 | CSB-EP517722EJV | Cusabio

Alternative Name(s): Fetidin (Hemolysin) (Lysenin-3) (efL3) (LRP-2)

Gene Names: N/A

Research Areas: Others

Organism: Eisenia fetida (Red wiggler worm)

AA Sequence: MSSRAGIAEGYEQIEVDVVAVWKEGYVYENRGSTSVEQKIKITKGMRNLNSETKTLTASHSIGSTISTGDLFEIATVDVSYSYSHEESQVSMTETEVYESKEIEHTITIPPTSKFTRWQLNADVGGADIEYMYLIDEVTPIGGTLSIPQVIKSRAKILVGREIYLGETEIRIKHADRKEYMTVVSRKSWPAATLGHSKLYKFVLYEDMYGFRIKTLNTMYSGYEYAYSSDQGGIYFDQGSDNPKQRWAINKSLPLRHGDVVTFMNKYFTRSGLCYYDGPATDVYCLDKREDKWILEVVKP

Source: E.coli

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Expression Region: 1-300aa

Sequence Info: Full Length

MW: 41.6 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Pore-forming toxin that specifically binds sphingomyelin in the plasma membrane of various cells. Has hemolytic activity. It also has antibacterial activities against B.megaterium.

Reference: "Relationship between hemolytic molecules in Eisenia fetida earthworms." Prochazkova P., Silerova M., Felsberg J., Joskova R., Beschin A., De Baetselier P., Bilej M. Dev. Comp. Immunol. 30:381-392(2006)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Pore-forming toxin that specifically binds sphingomyelin in the plasma membrane of various cells (By similarity). Has hemolytic activity. It also has antibacterial activities against B.megaterium.

Involvement in disease:

Subcellular Location: Secreted, Target cell membrane

Protein Families: Lysenin family

Tissue Specificity: Expressed by coelomocytes.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: O18425

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A