Recombinant Dendroaspis angusticeps Fasciculin-2 (Fas-2) | CSB-EP314272DBG

Recombinant Dendroaspis angusticeps Fasciculin-2 (Fas-2) | CSB-EP314272DBG | Cusabio

Alternative Name(s): Short name:Fas-2 Short name:Fas2 Alternative name(s): Acetylcholinesterase toxin F-VII Fasciculin-II Short name:FAS-II Toxin TA1

Gene Names: Fas-2

Research Areas: Others

Organism: Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)

AA Sequence: TMCYSHTTTSRAILTNCGENSCYRKSRRHPPKMVLGRGCGCPPGDDNLEVKCCTSPDKCNY

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-61aa

Sequence Info: Full Length

MW: 22.8 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Interferes with neuromuscular transmission by inhibiting the enzyme acetylcholinesterase (AChE) present at the neuromuscular junction. It selectively binds and inhibits with a 1:1 stoichiometry the mammalian and electric fish AChE at picomolar concentrations. It is highly specific for the peripheral site of AChE and blocks the entry of acetylcholine into the active site of the enzyme (through the Met-33 residue), thereby preventing its breakdown. It has been called fasciculin since after injection into mice it causes severe, generalized and long-lasting (5-7 hours) fasciculations.

Reference: "Snake venom toxins. The purification and amino acid sequence of toxin F-VII from Dendroaspis angusticeps venom."Viljoen C.C., Botes D.P.J. Biol. Chem. 248:4915-4919(1973)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Interferes with neuromuscular transmission by inhibiting the enzyme acetylcholinesterase (AChE) present at the neuromuscular junction. It selectively binds and inhibits with a 1

Involvement in disease:

Subcellular Location: Secreted

Protein Families: Snake three-finger toxin family, Short-chain subfamily, Acn-esterase inhibitor sub-subfamily

Tissue Specificity: Expressed by the venom gland.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P0C1Z0

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A