Recombinant Danio rerio D-2-hydroxyglutarate dehydrogenase, mitochondrial (d2hgdh) | CSB-EP374600DIL

Recombinant Danio rerio D-2-hydroxyglutarate dehydrogenase, mitochondrial (d2hgdh) | CSB-EP374600DIL | Cusabio

Alternative Name(s): d2hgdh; zgc:158661D-2-hydroxyglutarate dehydrogenase; mitochondrial; EC 1.1.99.-

Gene Names: d2hgdh

Research Areas: Others

Organism: Danio rerio (Zebrafish) (Brachydanio rerio)

AA Sequence: ANPANPSAAPPRLPFSRVTQEDLSFFRALLPGRTITDPDLLKSSNVDWLKTVQGSSDVLLRPKTTEGVSQILRYCNERNLAVCPQGGNTGLVGGSVPVFDEIILSTSLMNQVFAFDNISGILTCQAGCVLENLSHYLEERDFIMPLDLGAKGSCHIGGNVSTNAGGLRLLRYGSLRGTVLGLEVVLADGHVLNCLATLRKDNTGYDLKQLFIGSEGTLGVITAVSILCPRKPKAVNVAFLGCSSFQQLLETFQCCRGMLGEILSAFEFLDASCMNLLEKHLKLTNPITECPFYIVIETAGSNATHDEEKLHQFLEEVMTSSLVTDGTVATEATKIKALWSLRERVTEALTHEGYTYKYDISLPVEKIYDLVQDMRRHLGGMAKNVVGYGHVGDGNLHLNITSPSKDFDLLAAIEPYVYEWTSQWKGSISAEHGLGLKKRNYIYYSKPSEAVALMGSIKAMLDPKGILNPYKTLPDNIN

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 56-533aa

Sequence Info: Full Length of Mature Protein

MW: 68.3 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.

Reference: NIH - Zebrafish Gene Collection (ZGC) project

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.

Involvement in disease:

Subcellular Location: Mitochondrion

Protein Families: FAD-binding oxidoreductase/transferase type 4 family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: A1L258

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A