Recombinant Colletotrichum capsici Cutinase (CUTA) | CSB-EP319946CNE

Recombinant Colletotrichum capsici Cutinase (CUTA) | CSB-EP319946CNE | Cusabio

Alternative Name(s): Cutin hydrolase

Gene Names: CUTA

Research Areas: Cell Biology

Organism: Colletotrichum capsici (Anthracnose fungus)

AA Sequence: APVEVGLDTGVANLEARQSSTRNELESGSSSNCPKVIYIFARASTEPGNMGISAGPIVADALESRYGASQVWVQGVGGPYSADLASNFIIPEGTSRVAINEAKRLFTLANTKCPNSAVVAGGYSQGTAVMASSISELSSTIQNQIKGVVLSAITKNLQNLGRIPNFSTSKTEVYCALADAVCYGTLFILPAHFLYQADAATSAPRFLAARIG

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 17-228aa

Sequence Info: Full Length of Mature Protein

MW: 35.0 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of the fungal infection.

Reference: "Structure of cutinase gene, cDNA, and the derived amino acid sequence from phytopathogenic fungi." Ettinger W.F., Thukral S.K., Kolattukudy P.E. Biochemistry 26:7883-7892(1987)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P10951

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A