Recombinant Chicken Collagen alpha-1 (XII) chain (COL12A1) , partial | CSB-EP005719CH
- 13 - 23 Working Days
Recombinant Chicken Collagen alpha-1 (XII) chain (COL12A1) , partial | CSB-EP005719CH | Cusabio
Alternative Name(s): Fibrochimerin
Gene Names: COL12A1
Research Areas: Others
Organism: Gallus gallus (Chicken)
AA Sequence: DLVFLVDGSWSVGRNNFRYILDFMVALVSAFDIGEEKTRVGVVQYSSDTRTEFNLNQYFRRSDLLDAIKRIPYKGGNTMTGEAIDYLVKNTFTESAGARKGFPKVAIVITDGKAQDEVEIPARELRNIGVEVFSLGIKAADAKELKLIASQPSLKHVFNVANFDGIVDIQNEI
Tag Info: N-terminal 6xHis-tagged
Expression Region: 139-311aa
Sequence Info: Partial
MW: 23.3 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.
Reference: Type XII collagen. A large multidomain molecule with partial homology to type IX collagen.Gordon M.K., Gerecke D.R., Dublet B., van der Rest M., Olsen B.R.J. Biol. Chem. 264:19772-19778(1989)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.
Involvement in disease:
Subcellular Location: Secreted, extracellular space, extracellular matrix
Protein Families: Fibril-associated collagens with interrupted helices (FACIT) family
Tissue Specificity: Type XII collagen is present in tendons, ligaments, perichondrium, and periosteum, all dense connective tissues containing type I collagen.
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P13944
HGNC Database Link: N/A
UniGene Database Link: UniGene
KEGG Database Link: N/A
STRING Database Link: STRING
OMIM Database Link: N/A