Recombinant Burkholderia cenocepacia UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC) | CSB-EP451740BXS

Recombinant Burkholderia cenocepacia UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC) | CSB-EP451740BXS | Cusabio

Alternative Name(s): UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase

Gene Names: lpxC

Research Areas: Others

Organism: Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))

AA Sequence: MLKQRTIKSIVKTVGIGVHSGRKIELTLRPAAPGTGIVFSRVDLPTPVDIPASAMSIGDTRLASVLQKDGVRVSTVEHLMSACAGLGIDNLYVDVTAEEIPIMDGSAATFVFLIQSAGIEEQNAPKRFIKVKKPVEIRDGDKFARLDPYFGFKLKFSIDFRHPAVDKTGQELEVDFATTSYVREIARARTFGFAHEAEMLREIGLARGGSMDNAIVLDEYRILNNDGLRYDDEFVKHKMLDAIGDLYVIGHPLLASYTAYKSGHGLNNALLRELLAHEDAYEIVTFDDPQAAPKGFAFDAQTAFA

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged and C-terminal Myc-tagged

Expression Region: 1-305aa

Sequence Info: Full Length

MW: 51 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.

Reference: "The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients." Holden M.T., Seth-Smith H.M., Crossman L.C., Sebaihia M., Bentley S.D., Cerdeno-Tarraga A.M., Thomson N.R., Bason N., Quail M.A., Sharp S., Cherevach I., Churcher C., Goodhead I., Hauser H., Holroyd N., Mungall K., Scott P., Walker D. Parkhill J. J. Bacteriol. 191:261-277(2009)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.

Involvement in disease:

Subcellular Location:

Protein Families: LpxC family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: B4E5Y5

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A