Recombinant Bryonia dioica Ribosome-inactivating protein bryodin I, partial | CSB-YP339327BMR

Recombinant Bryonia dioica Ribosome-inactivating protein bryodin I, partial | CSB-YP339327BMR | Cusabio

Alternative Name(s): BD1 rRNA N-glycosidase

Gene Names: N/A

Research Areas: Others

Organism: Bryonia dioica (Red bryony) (Bryonia cretica subsp. dioica)

AA Sequence: DVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPLLRSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFKDAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTASSAASALLVLIQSTAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIASTNNGQFESPVVLIDGNNQRVS

Source: Yeast

Tag Info: N-terminal 6xHis-tagged

Expression Region: 24-247aa

Sequence Info: Partial

MW: 26.6 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells.

Reference: "Characterization of ribosome-inactivating proteins isolated from Bryonia dioica and their utility as carcinoma-reactive immunoconjugates." Siegall C.B., Gawlak S.L., Chace D., Wolff E.A., Mixan B., Marquardt H.Bioconj. Chem. 5:423-429(1994)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells.

Involvement in disease:

Subcellular Location:

Protein Families: Ribosome-inactivating protein family, Type 1 RIP subfamily

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P33185

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A