Recombinant Bovine Beta-1, 4-galactosyltransferase 1 (B4GALT1), partial | CSB-YP002513BO

Recombinant Bovine Beta-1, 4-galactosyltransferase 1 (B4GALT1), partial | CSB-YP002513BO | Cusabio

Alternative Name(s): UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1 UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

Gene Names: B4GALT1

Research Areas: Developmental Biology

Organism: Bos taurus (Bovine)

AA Sequence: MKFREPLLGGSAAMPGASLQRACR

Source: Yeast

Tag Info: N-terminal GST-tagged

Expression Region: 1-24aa

Sequence Info: Cytoplasmic Domain

MW: 29.5 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.

Reference: "Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library." Shaper N.L., Shaper J.H., Meuth J.L., Fox J.L., Chang H., Kirsch I.R., Hollis G.F. Proc. Natl. Acad. Sci. U.S.A. 83:1573-1577(1986)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.; FUNCTION

Involvement in disease:

Subcellular Location: Isoform Long: Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein, Cell membrane, Single-pass type II membrane protein, Cell surface, Cell projection, filopodium, Note=Found in trans cisternae of Golgi, B4GALT1 cell surface expression is regulated by UBE2Q1 (By similarity), SUBCELLULAR LOCATION: Isoform Short: Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein, Note=Found in trans cisternae of Golgi, SUBCELLULAR LOCATION: Processed beta-14-galactosyltransferase 1: Secreted

Protein Families: Glycosyltransferase 7 family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P08037

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A