Recombinant Bitis gabonica Thrombin-like enzyme gabonase, partial | CSB-EP314359BGW

Recombinant Bitis gabonica Thrombin-like enzyme gabonase, partial | CSB-EP314359BGW | Cusabio

Alternative Name(s): Fibrinogen-clotting enzyme Snake venom serine protease

Gene Names: N/A

Research Areas: Others

Organism: Bitis gabonica (Gaboon adder) (Gaboon viper)

AA Sequence: VVGGAECKIDGHRCLALLY

Source: E.coli

Tag Info: Tag-Free

Expression Region: 1-19aa

Sequence Info: Partial

MW: 2 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Thrombin-like snake venom serine protease. Releases both fibrinopeptides A and B from fibrinogen (FGA and FGB) to form fibrin clots. Also activates factor XIII (F13A). The activity of the enzyme is stabilized by calcium ion.

Reference: "Thrombin-like enzyme from the venom of Bitis gabonica. Purification, properties, and coagulant actions." Pirkle H., Theodor I., Miyada D., Simmons G. J. Biol. Chem. 261:8830-8835(1986)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Thrombin-like snake venom serine protease. Releases both fibrinopeptides A and B from fibrinogen (FGA and FGB) to form fibrin clots. Also activates factor XIII (F13A). The activity of the enzyme is stabilized by calcium ion.

Involvement in disease:

Subcellular Location: Secreted

Protein Families: Peptidase S1 family, Snake venom subfamily

Tissue Specificity: Expressed by the venom gland.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P0C577

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A