Recombinant Bacillus thuringiensis subsp. tenebrionis Pesticidal crystal protein cry3Aa (cry3Aa) | CSB-EP358337BDGa1
- 13 - 23 Working Days
Recombinant Bacillus thuringiensis subsp. tenebrionis Pesticidal crystal protein cry3Aa (cry3Aa) | CSB-EP358337BDGa1 | Cusabio
Alternative Name(s): 73KDA crystal protein Crystaline entomocidal protoxin Insecticidal delta-endotoxin CryIIIA(a)
Gene Names: cry3Aa
Research Areas: Others
Organism: Bacillus thuringiensis subsp. tenebrionis
AA Sequence: DSSTTKDVIQKGISVVGDLLGVVGFPFGGALVSFYTNFLNTIWPSEDPWKAFMEQVEALMDQKIADYAKNKALAELQGLQNNVEDYVSALSSWQKNPVSSRNPHSQGRIRELFSQAESHFRNSMPSFAISGYEVLFLTTYAQAANTHLFLLKDAQIYGEEWGYEKEDIAEFYKRQLKLTQEYTDHCVKWYNVGLDKLRGSSYESWVNFNRYRREMTLTVLDLIALFPLYDVRLYPKEVKTELTRDVLTDPIVGVNNLRGYGTTFSNIENYIRKPHLFDYLHRIQFHTRFQPGYYGNDSFNYWSGNYVSTRPSIGSNDIITSPFYGNKSSEPVQNLEFNGEKVYRAVANTNLAVWPSAVYSGVTKVEFSQYNDQTDEASTQTYDSKRNVGAVSWDSIDQLPPETTDEPLEKGYSHQLNYVMCFLMQGSRGTIPVLTWTHKSVDFFNMIDSKKITQLPLVKAYKLQSGASVVAGPRFTGGDIIQCTENGSAATIYVTPDVSYSQKYRARIHYASTSQITFTLSLDGAPFNQYYFDKTINKGDTLTYNSFNLASFSTPFELSGNNLQIGVTGLSAGDKVYIDKIEFIPVN
Tag Info: N-terminal 6xHis-tagged and C-terminal Myc-tagged
Expression Region: 58-644aa
Sequence Info: Full Length of Mature Protein
MW: 72 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera.
Reference: "Nucleotide sequence of a gene encoding an insecticidal protein of Bacillus thuringiensis var. tenebrionis toxic against Coleoptera." Hoefte H., Seurinck J., Houtven A.V., Vaeck M. Nucleic Acids Res. 15:7183-7183(1987)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera.
Involvement in disease:
Protein Families: Delta endotoxin family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P0A379
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A