Recombinant Bacillus anthracis Thymidylate kinase (tmk) | CSB-EP007226BQG

Recombinant Bacillus anthracis Thymidylate kinase (tmk) | CSB-EP007226BQG | Cusabio

Alternative Name(s): tmk; BAMEG_0039Thymidylate kinase; EC 2.7.4.9; dTMP kinase

Gene Names: tmk

Research Areas: Others

Organism: Bacillus anthracis (strain CDC 684 / NRRL 3495)

AA Sequence: MKGLFVTIEGPEGSGKTTLIQSLLPYFEQKEQKVMATREPGGIAISEDIRTILHKQEYTMMEARTEALLYAAARRQHLVEKVMPALNEDYLVLCDRFIDSSLAYQGYARGLGMDKVFEINRFATEDCMPSLTIYLDIEPEVGLARIAKDAGREVNRLDMEDISFHKRVREGYLQVVERFSDRIVLVNADQPMEKLIEEVIQVIEDKLL

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 1-208aa

Sequence Info: Full Length

MW: 27.8 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.

Reference: "Genome sequence of Bacillus anthracis str. CDC 684."Dodson R.J., Munk A.C., Brettin T., Bruce D., Detter C., Tapia R., Han C., Sutton G., Sims D.Submitted (OCT-2008)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.

Involvement in disease:

Subcellular Location:

Protein Families: Thymidylate kinase family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: C3LJ02

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: N/A

OMIM Database Link: N/A