Recombinant Arabidopsis thaliana UDP-glycosyltransferase 78D1 (UGT78D1) | CSB-EP871008DOA

Recombinant Arabidopsis thaliana UDP-glycosyltransferase 78D1 (UGT78D1) | CSB-EP871008DOA | Cusabio

Alternative Name(s): Flavonol-3-O-glucoside L-rhamnosyltransferase UDP-rhamnose:flavonol 3-O-glucoside rhamnosyltransferase

Gene Names: UGT78D1

Research Areas: Others

Organism: Arabidopsis thaliana (Mouse-ear cress)

AA Sequence: MTKFSEPIRDSHVAVLAFFPVGAHAGPLLAVTRRLAAASPSTIFSFFNTARSNASLFSSDHPENIKVHDVSDGVPEGTMLGNPLEMVELFLEAAPRIFRSEIAAAEIEVGKKVTCMLTDAFFWFAADIAAELNATWVAFWAGGANSLCAHLYTDLIRETIGLKDVSMEETLGFIPGMENYRVKDIPEEVVFEDLDSVFPKALYQMSLALPRASAVFISSFEELEPTLNYNLRSKLKRFLNIAPLTLLSSTSEKEMRDPHGCFAWMGKRSAASVAYISFGTVMEPPPEELVAIAQGLESSKVPFVWSLKEKNMVHLPKGFLDRTREQGIVVPWAPQVELLKHEAMGVNVTHCGWNSVLESVSAGVPMIGRPILADNRLNGRAVEVVWKVGVMMDNGVFTKEGFEKCLNDVFVHDDGKTMKANAKKLKEKLQEDFSMKGSSLENFKILLDEIVKV

Source: E.coli

Tag Info: N-terminal 10xHis-tagged

Expression Region: 1-453aa

Sequence Info: Full Length

MW: 55.6 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Acts as a UDP-rhamnose:flavonol-3-O-glucoside rhamnosyltransferase. Keampferol and quercitin are used as substrates. Possesses low quercetin 3-O-glucosyltransferase activity in vitro.

Reference: "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana." Li Y., Baldauf S., Lim E.K., Bowles D.J. J. Biol. Chem. 276:4338-4343(2001)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Acts as a UDP-rhamnose

Involvement in disease:

Subcellular Location:

Protein Families: UDP-glycosyltransferase family

Tissue Specificity: Expressed in leaves, flowers, siliques, and stems.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q9S9P6

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A