Recombinant Acinetobacter calcoaceticus Catechol 1, 2-dioxygenase | CSB-EP306913AWR

Recombinant Acinetobacter calcoaceticus Catechol 1, 2-dioxygenase | CSB-EP306913AWR | Cusabio

Alternative Name(s): Catechol 1,2-dioxygenase; EC 1.13.11.1; Fragment

Gene Names: N/A

Research Areas: Others

Organism: Acinetobacter calcoaceticus

AA Sequence: MNSQQIDMLVKQLN

Source: E.coli

Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Expression Region: 1-14aa

Sequence Info: Full Length

MW: 21.7 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance:

Reference: "Growth on phenol at chemostress levels amplifies the expression of the phenol degradation pathway in Acinetobacter calcoaceticus." Benndorf D., Loffhagen N., Hartig C., Babel W. Eng. Life Sci. 4:38-42(2004)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families: Intradiol ring-cleavage dioxygenase family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P83715

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A