PROM1 Antibody | CSB-PA018751LA01HU

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SKU:
CSB-PA018751LA01HU
Availability:
3 to 7 Working Days
  • PROM1 Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, K562 whole cell lysate, LO2 whole cell lysate, HepG2 whole cell lysate<br />
 All lanes: PROM1 antibody at 4.8µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 98, 97, 94, 93, 95 kDa<br />
 Observed band size: 98 kDa<br />
  • IHC image of CSB-PA018751LA01HU diluted at 1:1200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-PA018751LA01HU diluted at 1:1200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
€315.80 - €488.70

Description

PROM1 Antibody | CSB-PA018751LA01HU | Cusabio

PROM1 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: PROM1

Aliases: Prominin-1 (Antigen AC133) (Prominin-like protein 1) (CD antigen CD133), PROM1, PROML1

Background: May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617) . Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: O43490

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Prominin-1 protein (508-792AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC

Tested Applications: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:1000-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:1000-1:2000

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Neuroscience

Research Areas: Neuroscience;Stem cells

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