PMEL Antibody | CSB-PA021324LA01HU

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CSB-PA021324LA01HU
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3 to 7 Working Days
  • PMEL Antibody
  • Western blot<br />
 All lanes: PMEL antibody at 14µg/ml + Jurkat whole cell lysate<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/10000 dilution<br />
 Predicted band size: 71, 61, 67 kDa<br />
 Observed band size: 71 kDa<br />
  • IHC image of CSB-PA021324LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
£252.64 - £390.96

Description

PMEL Antibody | CSB-PA021324LA01HU | Cusabio

PMEL Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: PMEL

Aliases: Melanocyte protein PMEL (ME20-M) (ME20M) (Melanocyte protein Pmel 17) (Melanocytes lineage-specific antigen GP100) (Melanoma-associated ME20 antigen) (P1) (P100) (Premelanosome protein) (Silver locus protein homolog) [Cleaved into: M-alpha (95 kDa melanocyte-specific secreted glycoprotein) (P26) (Secreted melanoma-associated ME20 antigen) (ME20-S) (ME20S) ; M-beta], PMEL, D12S53E PMEL17 SILV

Background: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P40967

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Melanocyte protein PMEL protein (25-467AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC

Tested Applications: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:200-1:500

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Tags & Cell Markers

Research Areas: Cancer;Tags & Cell Markers;Immunology;Metabolism;Signal transduction

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