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PKM Monoclonal Antibody | CSB-MA018072A1m

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CSB-MA018072A1m
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3 to 7 Working Days
  • PKM Monoclonal Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate<br />
 All lanes: PKM antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 58 kDa<br />
 Observed band size: 58 KDa<br />
 Exposure time: 1min
  • Western Blot<br />
 Positive WB detected in: Mouse Brain tissue, Mouse Skeldtal Muscle tissue<br />
 All lanes: PKM antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 58 kDa<br />
 Observed band size: 58 KDa<br />
 Exposure time: 5min
  • Western Blot<br />
 Positive WB detected in: MCF-7 whole cell lysate at 40µg, 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg<br />
 All lanes: PKM antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 58 kDa<br />
 Observed band size: 58 KDa<br />
 Exposure time: 5min
  • Western Blot<br />
 Positive WB detected in: MCF-7 whole cell lysate<br />
 All lanes: PKM antibody at 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000, 1:512000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 58 kDa<br />
 Observed band size: 58 KDa<br />
 Exposure time: 5min
  • IHC image of CSB-MA018072A1m diluted at 1:1000 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA018072A1m diluted at 1:1000 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA018072A1m diluted at 1:1000 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA018072A1m diluted at 1:1000 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA018072A1m diluted at 1:1000 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA018072A1m diluted at 1:1000 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA018072A1m diluted at 1:1000 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA018072A1m diluted at 1:1000 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA018072A1m diluted at 1:1000 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of A549 cells with CSB-MA018072A1m at 1:215, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of Hela cells with CSB-MA018072A1m at 1:215, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of HepG2 cells with CSB-MA018072A1m at 1:215, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunoprecipitating PKM in Hela whole cell lysate<br />
 Lane 1: Mouse control IgG (1µg) instead of CSB-MA018072A1m in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-MA018072A1m (5µg) + Hela whole cell lysate (500µg) <br />
 Lane 3: Hela whole cell lysate (10µg) <br />
  • Overlay histogram showing Hela cells stained with CSB-MA018072A1m (red line) at 1:50. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.
  • Overlay histogram showing HepG2 cells stained with CSB-MA018072A1m (red line) at 1:50. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.
€373.00 - €555.00
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Description

PKM Monoclonal Antibody | CSB-MA018072A1m | Cusabio

PKM Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Monoclonal Antibody

Target Name: PKM

Aliases: CTHBP antibody; Cytosolic thyroid hormone-binding protein antibody; KPYM_HUMAN antibody; OIP-3 antibody; Opa-interacting protein 3 antibody; p58 antibody; pkm antibody; PKM1 antibody; PKM2 antibody; Pyruvate kinase 2/3 antibody; Pyruvate kinase muscle isozyme antibody; Pyruvate kinase PKM antibody; THBP1 antibody; Thyroid hormone-binding protein 1 antibody; Tumor M2-PK antibody

Relevance: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Isotype: IgG1

Conjugate: Non-conjugated

Clone Number: 4C8E12

Uniport ID: P14618

Alternatives To SCBT: #N/A

Host Species: Mouse

Species Reactivity: Human, Mouse

Immunogen: Recombinant Human Pyruvate kinase PKM protein (2-531AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, FC, IP

Tested Applications: ELISA, WB, IHC, IF, FC, IP; Recommended dilution: WB: 1:4000-1:512000, IHC: 1:500-1:1000, IF: 1:150-1:300, FC: 1:50-1:100, IP: 1µl-4µl

Purification Method: >95%, Protein G purified

Dilution Ratio1: WB:1:4000-1:512000

Dilution Ratio2: IHC:1:500-1:1000

Dilution Ratio3: IF:1:150-1:300

Dilution Ratio4: FC:1:50-1:100

Dilution Ratio5: IP:1µl-4µl

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Immunology

Research Areas: Immunology

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