Phospho-VCL (Tyr822) Antibody | CSB-PA177557

Phospho-VCL (Tyr822) Antibody | CSB-PA177557 | Cusabio

Phospho-VCL (Tyr822) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: VCL

Aliases: Vinculin;

Background:

Actin filament (F-actin) -binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Subauste MC, et al. (2004) J Cell Biol 165, 371-81
Olsen JV, et al. (2010) Sci Signal 3, ra3
J rgensen C, et al. (2009) Science 326, 1502-9

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P18206

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Peptide sequence around phosphorylation site of Tyrosine 822 (S-G-Y (p) -R-I) derived from Human Vinculin.

Immunogen Species: Human

Applications: ELISA, WB

Tested Applications: ELISA, WB;WB:1:500-1:1000

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3:

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Isotype Loading Controls;Cardiovascular;Signal transduction