Phospho-SNAI1 (Ser246) Antibody | CSB-PA298000

Phospho-SNAI1 (Ser246) Antibody | CSB-PA298000 | Cusabio

Phospho-SNAI1 (Ser246) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: SNAI1

Aliases: SNAH; SNAI; Sna; Snail;

Background:

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA) -induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.

Okubo T., Cancer Res. 61:1338-1346 (2001) .
Twigg S.R., Hum. Genet. 105:320-326 (1999) .
Paznekas W.A., Genomics 62:42-49 (1999) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: O95863

Host Species: Rabbit

Species Reactivity: Human, Mouse

Immunogen: Peptide sequence around phosphorylation site of Serine 246 (T-F-S (p) -R-M) derived from Human SNAI1.

Immunogen Species: Human

Applications: ELISA, WB, IF

Tested Applications: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3: IF:1:100-1:200

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling;Cancer;Cardiovascular