Phospho-SMAD3 (Ser425) Antibody | CSB-PA898915

Phospho-SMAD3 (Ser425) Antibody | CSB-PA898915 | Cusabio

Phospho-SMAD3 (Ser425) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: SMAD3

Aliases: JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3

Background: Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Shi W, et al. J Cell Sci. 2007 Apr 1;120 (Pt 7) :1216-24
Seong HA, et al. J Biol Chem. 2007 Apr 20;282 (16) :12272-89
Wordinger RJ, et al. Invest Ophthalmol Vis Sci. 2007 Mar;48 (3) :1191-200
LeClair RJ, et al. Circ Res. 2007 Mar 30;100 (6) :826-33

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P84022

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S (p) ) derived from Human Smad3.

Immunogen Species: Human

Applications: ELISA, WB, IHC

Tested Applications: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:100

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3: IHC:1:50-1:100

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Epigenetics & Nuclear Signaling;Cancer;Metabolism;Signal transduction;Stem cells