Phospho-RUNX1 (Ser435) Antibody | CSB-PA110286

Phospho-RUNX1 (Ser435) Antibody | CSB-PA110286 | Cusabio

Phospho-RUNX1 (Ser435) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: RUNX1

Aliases: Acute myeloid leukemia 1 protein; CBFA2; CBFA2; alpha 2 subunit; RUNX1

Background:

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.

Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434 (1991) .
Sacchi N., Genes Chromosomes Cancer 11:226-236 (1994) .
Nucifora G., Blood 81:2728-2734 (1993) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q01196

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Peptide sequence around phosphorylation site of Serine 435 (S-N-S (p) -P-T) derived from Human AML1.

Immunogen Species: Human

Applications: ELISA, WB

Tested Applications: ELISA, WB;WB:1:500-1:1000

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3:

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Neuroscience

Research Areas: Epigenetics & Nuclear Signaling;Neuroscience;Cancer;Developmental biology;Stem cells