Phospho-PRKAA1/PRKAA2 (Ser487) Antibody | CSB-PA261465

Phospho-PRKAA1/PRKAA2 (Ser487) Antibody | CSB-PA261465 | Cusabio

Phospho-PRKAA1/PRKAA2 (Ser487) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: PRKAA1/PRKAA2

Aliases: AAPK1; AMPK alpha-1 chain; AMPK-alpha1; HMG-CoA redustase kinase; PRKAA1

Background:

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Kim JE, et al. (2005) J Proteome Res. 4 (4) : 1339-1346.
Woods A, et al. (2003) J Biol Chem. 278 (31) : 28434-28442.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q13131/P54646

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Peptide sequence around phosphorylation site of serine 487 (S-G- SP-V-S) derived from Human AMPKa1.

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3: IHC:1:50-1:100

Dilution Ratio4: IF:1:100-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Neuroscience

Research Areas: Neuroscience;Cancer;Cardiovascular;Metabolism;Signal transduction