Phospho-PKM (Ser37) Antibody | CSB-PA779576

Phospho-PKM (Ser37) Antibody | CSB-PA779576 | Cusabio

Phospho-PKM (Ser37) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: PKM

Aliases: PKM, PK3, OIP3, PK2

Background:

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Weiwei Yang1, Yanhua Zheng1, Yan Xia1, Haitao Ji,   "ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect." Nature Cell Biology (2012) |doi:10.1038/ncb2629
Received16 August 2012 Accepted24 October 2012 Published online25 November 2012

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P14618

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around phosphorylation site of serine 37 (I-D-S (p) -P-P) derived from Human PKM2.

Immunogen Species: Human

Applications: ELISA, WB, IF

Tested Applications: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3: IF:1:100-1:200

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Epigenetics & Nuclear Signaling;Cancer;Metabolism;Signal transduction