Phospho-NFE2L2 (S40) Antibody | CSB-RA614961A40phHU

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CSB-RA614961A40phHU
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3 to 7 Working Days
  • Phospho-NFE2L2 (S40) Antibody
  • Western Blot<br />
 Positive WB detected in:HepG2 whole cell lysate,293 whole cell lysate(treated with Calyculin A or EGF)<br />
 All lanes:Phospho-NFE2L2 antibody at 0.8µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 90 KDa<br />
 Observed band size: 90 KDa<br />
  • IHC image of CSB-RA008836A253phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of Hela cells with CSB-RA614961A40phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
€373.00 - €555.00

Description

Phospho-NFE2L2 (S40) Antibody | CSB-RA614961A40phHU | Cusabio

Phospho-NFE2L2 (S40) Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant Antibody

Target Names: NFE2L2

Aliases: Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor, erythroid derived 2, like 2, NFE2L2, NRF2

Background: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 2,00E+06

Uniport ID: Q16236

Modified: Epigenetics and Nuclear Signaling

species: Human

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human Phospho-NFE2L2 (S40)

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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