Phospho-MDM2 (Ser186/Ser188) Antibody | CSB-PA570037

Phospho-MDM2 (Ser186/Ser188) Antibody | CSB-PA570037 | Cusabio

Phospho-MDM2 (Ser186/Ser188) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: MDM2

Aliases: Oncoprotein Mdm2; p53-binding protein Mdm2; Ubiquitin-protein ligase E3 Mdm2;

Background:

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues

M Zhou, Blood, Mar 1995; 85: 1608 - 1614.
Susan M. Mendrysa, Mol. Cell. Biol., Mar 2000; 20: 2023 - 2030.
M. Saeed Sheikh, Cancer Res., Jul 1993; 53: 3226 - 3228.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q00987

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around phosphorylation site of Ser186/Ser188 (H-K-S (p) -D-S (p) -I-S) derived from Human MDM2.

Immunogen Species: Human

Applications: ELISA, IF

Tested Applications: ELISA, IF;IF:1:100-1:200

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: IF:1:100-1:200

Dilution Ratio3:

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Epigenetics & Nuclear Signaling;Cancer;Cell biology