Phospho-MAP2K1 (Ser221) Antibody | CSB-PA439786

Phospho-MAP2K1 (Ser221) Antibody | CSB-PA439786 | Cusabio

Phospho-MAP2K1 (Ser221) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: MAP2K1

Aliases: ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1

Background:

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Zebisch A, et al. (2006) Cancer Res; 66 (7) : 3401-8.
Luciano BS, et al. (2006) J Biol Chem; 279 (50) : 52117-23.
Wang X, et al. (2003) Oncogene; 22 (1) : 109-16.
Gopalbhai K, et al. (2003) J Biol Chem; 278 (10) : 8118-25.
Ling MT, et al. (2002) Oncogene; 21 (55) : 8498-505.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q02750

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Peptide sequence around phosphorylation site of serine 221 (A-N-S (p) -F-V) derived from Human MEK1.

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3: IHC:1:50-1:100

Dilution Ratio4: IF:1:100-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Signal transduction