Phospho-HIST1H1D (T179) Antibody | CSB-PA010379OA179phHU

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CSB-PA010379OA179phHU
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  • Phospho-HIST1H1D (T179) Antibody
  • Immunofluorescence staining of Hela cells with CSB-PA010379OA179phHU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1D (CSB-PA010379OA179phHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
$393.00 - $617.64

Description

Phospho-HIST1H1D (T179) Antibody | CSB-PA010379OA179phHU | Cusabio

Phospho-HIST1H1D (T179) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: HIST1H1D

Aliases: Histone H1.3 (Histone H1c) (Histone H1s-2), HIST1H1D, H1F3

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P16402

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Phospho-Thr (179) derived from Human Histone H1.3

Immunogen Species: Human

Applications: ELISA, IF, ChIP

Tested Applications: ELISA, IF, ChIP; Recommended dilution: IF:1:20-1:200

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: IF:1:20-1:200

Dilution Ratio3:

Dilution Ratio4:

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Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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