Phospho-HIST1H1B (T154) Antibody | CSB-PA010377PA154phHU

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SKU:
CSB-PA010377PA154phHU
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3 to 7 Working Days
  • Phospho-HIST1H1B (T154) Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate<br />
 All lanes: HIST1H1B antibody at 1.5µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 23 kDa<br />
 Observed band size: 32 kDa<br />
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 100nM calyculin A for 60min) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1B (CSB-PA010377PA154phHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
  • Immunofluorescence staining of HepG2 cells with CSB-PA010377PA154phHU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
£262.00 - £411.76

Description

Phospho-HIST1H1B (T154) Antibody | CSB-PA010377PA154phHU | Cusabio

Phospho-HIST1H1B (T154) Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: HIST1H1B

Aliases: Histone H1.5 (Histone H1a) (Histone H1b) (Histone H1s-3), HIST1H1B, H1F5

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P16401

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Phospho-Thr (154) derived from Human Histone H1.5

Immunogen Species: Human

Applications: ELISA, WB, IF, ChIP

Tested Applications: ELISA, WB, IF, ChIP; Recommended dilution: WB:1:200-1:2000, IF:1:50-1:200

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:200-1:2000

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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