Phospho-ERN1 (S724) Antibody | CSB-RA007795A724phHU

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SKU:
CSB-RA007795A724phHU
Availability:
3 to 7 Working Days
  • Phospho-ERN1 (S724) Antibody
  • Western Blot<br />
 Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate (treated with Calyculin A or EGF)<br />
 All lanes: Phospho-ERN1 antibody at 0.75µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 110 kDa<br />
 Observed band size: 110 kDa<br />
  • Immunofluorescence staining of Hela cells with CSB-RA007795A724phHU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
€373.00 - €555.00

Description

Phospho-ERN1 (S724) Antibody | CSB-RA007795A724phHU | Cusabio

Phospho-ERN1 (S724) Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant Antibody

Target Names: ERN1

Aliases: Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, hIRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, Endoribonuclease, ERN1

Background: Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation (PubMed:9637683, PubMed:11175748, PubMed:12637535). The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis (PubMed:24508390).

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 1F12

Uniport ID: O75460

Modified: Signal Transduction

species: Human

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human Phospho-ERN1 (S724)

Tested Applications: ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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