Phospho-EIF4E (Ser209) Antibody | CSB-PA935753

Phospho-EIF4E (Ser209) Antibody | CSB-PA935753 | Cusabio

Phospho-EIF4E (Ser209) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: EIF4E

Aliases: mRNA cap-binding protein; eIF-4F 25 kDa subunit;

Background:

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Li BD, et al. (1998) Ann Surg; 227 (5) : 756-763
Altmann M, et al. (1989) Nucleic Acids Res; 17 (18) : 7520
De Gregorio E, et al. (2001) RNA; 7 (1) : 106-113
Gu W, et al. (2004) Nucleic Acids Res; 32 (15) : 4448-4461

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P06730

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Peptide sequence around phosphorylation site of serine 209 (S-G-S (p) -T-T) derived from Human eIF4E.

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3: IHC:1:50-1:100

Dilution Ratio4: IF:1:100-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling