Phospho-EIF4E (S209) Antibody | CSB-RA007556A209phHU

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SKU:
CSB-RA007556A209phHU
Availability:
3 to 7 Working Days
  • Phospho-EIF4E (S209) Antibody
  • Western Blot<br />
 Positive WB detected in:293 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or not)<br />
 All lanes:Phospho-EIF4E antibody at 1.2µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 25 KDa<br />
 Observed band size: 25 KDa<br />
  • IHC image of CSB-RA007556A209phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
€373.00 - €555.00

Description

Phospho-EIF4E (S209) Antibody | CSB-RA007556A209phHU | Cusabio

Phospho-EIF4E (S209) Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant Antibody

Target Names: EIF4E

Aliases: Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F

Background: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 1F3

Uniport ID: P06730

Modified: Epigenetics and Nuclear Signaling

species: Human

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human Phospho-EIF4E (S209)

Tested Applications: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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