Phospho-CHUK/IKBKB (Ser176/177) Antibody | CSB-PA983649

Phospho-CHUK/IKBKB (Ser176/177) Antibody | CSB-PA983649 | Cusabio

Phospho-CHUK/IKBKB (Ser176/177) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: CHUK/IKBKB

Aliases: FLJ40509; I-kappa-B kinase; IKBKB; kinase beta; NFKBIKB

Background:

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3.

Chandrakesan P, et al. (2010) J Biol Chem 285, 33485-98
Hinz M, et al. (2010 Mol Cell 40, 63-74
Choudhary S, Lu M, Cui R, Brasier AR (2007) Mol Endocrinol 21, 2203-17

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: O15111/O14920

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Peptide sequence around phosphorylation site of serine 176/177 (Q-G-S (p) -L-C) derived from Human IKK-alpha/beta.

Immunogen Species: Human

Applications: ELISA, WB

Tested Applications: ELISA, WB;WB:1:500-1:1000

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3:

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Cancer;Cardiovascular;Cell biology;Immunology;Signal transduction