Phospho-CCNB1 (Ser147) Antibody | CSB-PA059325

Phospho-CCNB1 (Ser147) Antibody | CSB-PA059325 | Cusabio

Phospho-CCNB1 (Ser147) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: CCNB1

Aliases:

Background: The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470.
Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685.
Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P14635

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around phosphorylation site of Serine 147 (A-F-S (p) -D-V) derived from Human Cyclin B1.

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3: IHC:1:50-1:100

Dilution Ratio4: IF:1:100-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Epigenetics & Nuclear Signaling;Cancer;Cell biology