Phospho-BTK (Tyr223) Antibody | CSB-PA142166

Phospho-BTK (Tyr223) Antibody | CSB-PA142166 | Cusabio

Phospho-BTK (Tyr223) Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: BTK

Aliases: AGMX1; ATK; kinase Btk; BPK;

Background:

Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK.

Sulekha Verma, J. Biol. Chem., Feb 2001; 276: 4671 - 4676.
Yan M. Li, Cancer Res., Nov 2003; 63: 7630 - 7633.
Malti Nikrad, Mol. Cancer Ther., Mar 2005; 4: 443 - 449.
Feng Dong, Infect. Immun., Mar 2005; 73: 1861 - 1864.
Hiroo Ueno, Mol. Biol. Cell, Feb 2

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q06187

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Peptide sequence around phosphorylation site of tyrosine 223 (A-L-Y (p) -D-Y) derived from Human BTK.

Immunogen Species: Human

Applications: ELISA, WB

Tested Applications: ELISA, WB;WB:1:500-1:1000

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3:

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Immunology;Signal transduction