Phospho-ATF4 (Ser219) Antibody | CSB-PA172388

Phospho-ATF4 (Ser219) Antibody | CSB-PA172388 | Cusabio

Phospho-ATF4 (Ser219) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: ATF4

Aliases: ATF4; CREB-2; DNA-binding protein TAXREB67; TXREB;

Background:

ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA bindingprotein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2) . The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Sato T., Shimotohno K. J. Virol. 65:1420-1426 (1991)
Leiden J.M. Proc. Natl. Acad. Sci. U.S.A. 89:4820-4824 (1992)
Bridgeman A.M. Wright H. Nature 402:489-495 (1999)

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P18848

Host Species: Rabbit

Species Reactivity: Human, Mouse

Immunogen: Peptide sequence around phosphorylation site of Serine 219 (N-D-S (p) -G-T) derived from Human ATF-4.

Immunogen Species: Human

Applications: ELISA, WB

Tested Applications: ELISA, WB;WB:1:500-1:1000

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3:

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling