Phospho-ATF2 (T71) Antibody | CSB-RA002270A71phHU

(No reviews yet) Write a Review
SKU:
CSB-RA002270A71phHU
Availability:
3 to 7 Working Days
  • Phospho-ATF2 (T71) Antibody
  • Western Blot<br />
 Positive WB detected in:293 whole cell lysate,A549 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF)<br />
 All lanes:Phospho-ATF2 antibody at 1.015µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 70 KDa<br />
 Observed band size: 70 KDa<br />
  • Immunofluorescence staining of A549 cells(treated with 100mM EGF for 20min) with CSB-RA002270A71phHU at 1:63,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
£298.40 - £444.00

Description

Phospho-ATF2 (T71) Antibody | CSB-RA002270A71phHU | Cusabio

Phospho-ATF2 (T71) Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant Antibody

Target Names: ATF2

Aliases: Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2, HB16, Histone acetyltransferase ATF2, cAMP response element-binding protein CRE-BP1, ATF2, CREB2, CREBP1

Background: Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 4F2

Uniport ID: P15336

Modified: Epigenetics and Nuclear Signaling

species: Human

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human Phospho-ATF2 (T71)

Tested Applications: ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

View AllClose

0 Reviews

View AllClose