PHF6 Antibody | CSB-PA017917LA01HU

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CSB-PA017917LA01HU
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  • PHF6 Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate<br />
 All lanes: PHF6 antibody at 2.8µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 42, 36, 38 kDa<br />
 Observed band size: 42 kDa<br />
  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA017917LA01HU at dilution of 1:100
  • Immunoprecipitating PHF6 in Jurkat whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA017917LA01HU in Jurkat whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA017917LA01HU (8µg) + Jurkat whole cell lysate (500µg) <br />
 Lane 3: Jurkat whole cell lysate (10µg) <br />
  • Immunofluorescence staining of Hela cells with CSB-PA017917LA01HU at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
€315.80 - €488.70

Description

PHF6 Antibody | CSB-PA017917LA01HU | Cusabio

PHF6 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: PHF6

Aliases: PHD finger protein 6 (PHD-like zinc finger protein), PHF6, CENP-31 KIAA1823

Background: Transcriptional regulator that associates with ribosomal RNA promoters and suppresses ribosomal RNA (rRNA) transcription.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q8IWS0

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human PHD finger protein 6 protein (2-312AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, IP

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:500, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:50-1:500

Dilution Ratio5: IP:1:200-1:2000

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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