PHB Antibody | CSB-PA017885LA01HU

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  • PHB Antibody
  • IHC image of CSB-PA017885LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Western Blot<br />
 Positive WB detected in: Raji whole cell lysate<br />
 All lanes: PHB antibody at 4.5µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 30, 18 kDa<br />
 Observed band size: 30 kDa<br />
  • Immunoprecipitating PHB in HEK293 whole cell lysate<br />
 Lane 1: Rabbit control IgG (1µg) instead of CSB-PA017885LA01HU in HEK293 whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA017885LA01HU (8µg) + HEK293 whole cell lysate (500µg) <br />
 Lane 3: HEK293 whole cell lysate (10µg) <br />
$378.96 - $586.44


PHB Antibody | CSB-PA017885LA01HU | Cusabio

PHB Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: PHB

Aliases: Prohibitin, PHB

Background: Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P35232

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Prohibitin protein (1-272AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IP

Tested Applications: ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:200-1:500

Dilution Ratio4: IP:1:200-1:2000

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Epigenetics & Nuclear Signaling;Cancer;Cell biology;Tags & Cell Markers;Metabolism

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