PD-L2 Monoclonal Antibody | CSB-MA017667A0m

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SKU:
CSB-MA017667A0m
Availability:
3 to 7 Working Days
  • PD-L2 Monoclonal Antibody
  • Western Blot<br /> Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate <br /> All lanes PD-L2 antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to mouse IgG at 1/50000 dilution<br /> Predicted band size: 31, 21 KDa<br /> Observed band size: 45-50 KDa<br /> Exposure time：5min
  • Western Blot<br /> Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg All lanes: PD-L2 antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to mouse IgG at 1/50000 dilution<br /> Predicted band size: 31, 21 KDa<br /> Observed band size: 45-50 KDa<br /> Exposure time：5min
  • IHC image of CSB-MA017667A0m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of HepG2 cells with CSB-MA017667A0m at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of Hela cells with CSB-MA017667A0m at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of Raji cells with CSB-MA017667A0m at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of U251 cells with CSB-MA017667A0m at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Overlay histogram showing 293 cells transfected with PD-L2 stained with CSB-MA017667A0m (red line) . The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (2µg/1*106cells) for 1 h at 4°C. The secondary antibody used was R-PE-conjugated Goat Anti-Mouse IgG (H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (2µg/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • Immunoprecipitating PD-L2 in Hela whole cell lysate <br /> Lane 1: Mouse control IgG instead of CSB-MA017667A0m in Hela whole cell lysate. Lane 2: CSB-MA017667A0m (2µl) + Hela whole cell lysate (500µg) <br /> Lane 3: Hela whole cell lysate (20µg) <br /> For western blotting, the blot was detected with CSB-MA017667A0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 <br />
€373.00 - €555.00

Description

PD-L2 Monoclonal Antibody | CSB-MA017667A0m | Cusabio

PD-L2 Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Monoclonal Antibody

Target Name: PD-L2

Aliases: B7 dendritic cell molecule antibody; B7-DC antibody; B7DC antibody; bA574F11.2 antibody; Btdc antibody; Butyrophilin B7 DC antibody; Butyrophilin B7-DC antibody; Butyrophilin B7DC antibody; CD 273 antibody; CD273 antibody; CD273 antigen antibody; MGC142238 antibody; MGC142240 antibody; PD 1 ligand 2 antibody; PD L2 antibody; PD-1 ligand 2 antibody; PD-L2 antibody; PD1 ligand 2 antibody; PD1L2_HUMAN antibody; PDCD 1 ligand 2 antibody; PDCD1 ligand 2 antibody; PDCD1L2 antibody; Pdcd1lg2 antibody; PDL 2 antibody; PDL2 antibody; Programmed cell death 1 ligand 2 antibody; Programmed death ligand 2 antibody

Relevance: Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

Isotype: IgG2b

Conjugate: Non-conjugated

Clone Number: 7F11D11

Uniport ID: Q9BQ51

Alternatives To SCBT: #N/A

Host Species: Mouse

Species Reactivity: Human

Immunogen: Recombinant Human Programmed cell death 1 ligand 2 protein (21-118AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, FC, IP

Tested Applications: ELISA, WB, IHC, IF, FC, IP; Recommended dilution: WB: 1:5000-1:640000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200, IP:2µl-5µl

Purification Method: >95%, Protein A purified

Dilution Ratio1: WB: 1:5000-1:640000

Dilution Ratio2: IHC:1:50-1:200

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4: FC:1:50-1:200

Dilution Ratio5: IP:2µl-5µl

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Immunology

Research Areas: Immunology

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