PCNA Antibody | CSB-PA974334

PCNA Antibody | CSB-PA974334 | Cusabio

PCNA Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: PCNA

Aliases: cyclin; proliferating cell nuclear antigen;

Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Anje Sporbert, Nucleic Acids Res., Jun 2005; 33: 3521 - 3528.
Sean C. Gehen, Am J Physiol Lung Cell Mol Physiol, Mar 2007; 292: L716 - L724.
Basavaraj Bagewadi, J. Virol., Nov 2004; 78: 11890 - 11903.
T Iida, I Suetake, Genes Cells, Oct 2002; 7: 997 -

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P12004

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Synthesized peptide derived from human PCNA.

Immunogen Species: Human

Applications: ELISA, WB, IHC

Tested Applications: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

Purification Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:3000

Dilution Ratio3: IHC:1:50-1:100

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Isotype Loading Controls;Epigenetics & Nuclear Signaling;Cancer;Cell biology;Tags & Cell Markers