NUP98 Antibody | CSB-PA016209LA01HU

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SKU:
CSB-PA016209LA01HU
Availability:
3 to 7 Working Days
  • NUP98 Antibody
  • Western Blot<br />
 Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate<br />
 All lanes: NUP98 antibody at 3.2µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 198, 188, 98, 97, 196, 187 kDa<br />
 Observed band size: 105 kDa<br />
  • Immunofluorescence staining of A549 cells with CSB-PA016209LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Immunoprecipitating NUP98 in Jurkat whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA016209LA01HU in Jurkat whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA016209LA01HU (8µg) + Jurkat whole cell lysate (500µg) <br />
 Lane 3: Jurkat whole cell lysate (20µg) <br />
$378.96 - $586.44

Description

NUP98 Antibody | CSB-PA016209LA01HU | Cusabio

NUP98 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: NUP98

Aliases: Nuclear pore complex protein Nup98-Nup96 (EC 3.4.21.-) [Cleaved into: Nuclear pore complex protein Nup98 (98 kDa nucleoporin) (Nucleoporin Nup98) (Nup98) ; Nuclear pore complex protein Nup96 (96 kDa nucleoporin) (Nucleoporin Nup96) (Nup96) ], NUP98, ADAR2

Background: Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Nup98 and Nup96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P52948

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Nuclear pore complex protein Nup98-Nup96 protein (965-1110AA)

Immunogen Species: Human

Applications: ELISA, WB, IF, IP

Tested Applications: ELISA, WB, IF, IP; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4: IP:1:200-1:2000

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Epigenetics & Nuclear Signaling;Signal transduction

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