NFKBIA Antibody | CSB-PA015761LA01HU

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CSB-PA015761LA01HU
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  • NFKBIA Antibody
  • Western Blot<br />
 Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, Raji whole cell lysate<br />
 All lanes: NFKBIA antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 36 kDa<br />
 Observed band size: 36 kDa<br />
  • IHC image of CSB-PA015761LA01HU diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of HepG2 cells with CSB-PA015761LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
£252.64 - £390.96

Description

NFKBIA Antibody | CSB-PA015761LA01HU | Cusabio

NFKBIA Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: NFKBIA

Aliases: NF-kappa-B inhibitor alpha (I-kappa-B-alpha) (IkB-alpha) (IkappaBalpha) (Major histocompatibility complex enhancer-binding protein MAD3), NFKBIA, IKBA MAD3 NFKBI

Background: Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P25963

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human NF-kappa-B inhibitor alpha protein (27-317AA)

Immunogen Species: Homo sapiens (Human)

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:100-1:500, IF:1:100-1:500

Purification Method: >95%, Antigen Affinity purified & Affinity purified

Dilution Ratio1: WB:1:1000-1:5000

Dilution Ratio2: IHC:1:100-1:500

Dilution Ratio3: IF:1:100-1:500

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Signal Transduction

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