NFATC3 Antibody | CSB-PA615540LA01HU

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CSB-PA615540LA01HU
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  • NFATC3 Antibody
  • IHC image of CSB-PA615540LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Rat kidney tissue<br />
 All lanes: NFATC3 antibody at 4.6µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 116, 113, 78, 81 kDa<br />
 Observed band size: 116 kDa<br />
  • Immunofluorescence staining of Hela cells with CSB-PA615540LA01HU at 1:155, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
€315.80 - €488.70

Description

NFATC3 Antibody | CSB-PA615540LA01HU | Cusabio

NFATC3 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: NFATC3

Aliases: Nuclear factor of activated T-cells, cytoplasmic 3 (NF-ATc3) (NFATc3) (NFATx) (T-cell transcription factor NFAT4) (NF-AT4), NFATC3, NFAT4

Background: Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q12968

Host Species: Rabbit

Species Reactivity: Human, Rat

Immunogen: Recombinant Human Nuclear factor of activated T-cells, cytoplasmic 3 protein (887-1070AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:200-1:500

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Epigenetics & Nuclear Signaling;Signal transduction

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