NES Monoclonal Antibody | CSB-MA0157131A0m

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CSB-MA0157131A0m
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3 to 7 Working Days
  • NES Monoclonal Antibody
  • Western Blot<br />
 Positive WB detected in: U251 whole cell lysate, SH-SY5Y whole cell lysate<br />
 All lanes: NES antibody at 3µg/ml<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 178 kDa<br />
 Observed band size: 260 kDa<br />
  • Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-MA0157131A0m at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA0157131A0m at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human melanoma using CSB-MA0157131A0m at dilution of 1:100
  • Immunofluorescent analysis of Hela cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescent analysis of PC-3 cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescent analysis of U251 cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Overlay histogram showing U251 cells stained with CSB-MA0157131A0m (red line) . The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*10<sup>6</sup>cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • Overlay histogram showing Hela cells stained with CSB-MA0157131A0m (red line) . The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*10<sup>6</sup>cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10, 000 events was performed.
£298.40 - £444.00

Description

NES Monoclonal Antibody | CSB-MA0157131A0m | Cusabio

NES Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Monoclonal Antibody

Target Name: NES

Aliases: Nestin, NES, Nbla00170

Relevance: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Isotype: IgG1

Conjugate: Non-conjugated

Clone Number: 2C2F7

Uniport ID: P48681

Alternatives To SCBT: sc-23927/sc-71665/sc-73489

Host Species: Mouse

Species Reactivity: Human

Immunogen: Recombinant Human Nestin protein

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, FC

Tested Applications: ELISA, WB, IHC, IF, FC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: WB:1:500-1:5000

Dilution Ratio2: IHC:1:50-1:500

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4:

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Neuroscience

Research Areas: Neuroscience;Cancer;Cardiovascular;Developmental biology;Stem cells

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