Myc tag Monoclonal antibody | CSB-MA000041M0m

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3 to 7 Working Days
  • Myc tag Monoclonal antibody
  • WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6μg/ml<br />
 Lane 1: Recombinant Myc-tagged fusion protein at 50ng<br />
 Lane 2: Recombinant Myc-tagged fusion protein at 25ng<br />
 Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng<br />
 Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng<br />
 Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng<br />
 Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/50000 dilution<br />
 Predicted band size: 50 kd<br />
 Observed band size: 50 kd<br />
  • Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
  • Immunoprecipitating MYC-tag with transfected HEK293<br />
 Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br />
 Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg)<br />
 Lane 3: Transfected HEK293 whole cell lysate (10µg)<br />
€256.00 - €360.00
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Myc tag Monoclonal antibody | CSB-MA000041M0m | Cusabio

Myc tag Monoclonal antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Tag/Control Antibodies

Target Name: Myc tag

Aliases: bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64

Relevance: Myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein. It can help to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. Myc Tag also can be used to isolate protein complexes with multiple subunits. The Myc Tag Antibody is produced by the conjugation of a synthetic Myc tag peptide to KLH. Myc Tag Antibody can be used in various immunoassays, such as ELISA, Western blotting, immunoprecipitation, immunofluorescence, and more.

Isotype: IgG1

Conjugate: Non-conjugated

Clone Number:

Host Species: Mouse

Species Reactivity: All

Immunogen: EQKLISEEDL (Myc) synthetic peptide conjugate to KLH

Applications: ELISA, WB, IF, IP

Tested Applications: ELISA, WB, IF, IP; Recommended dilution: WB:1:500-1:5000, IF:1:100-1:300, IP:1:1000-1:1500

Purification Method: >95%, Protein G purified

Dilution Ratio1: WB:1:500-1:5000

Dilution Ratio2: IF:1:100-1:300

Dilution Ratio3: IP:1:1000-1:1500

Dilution Ratio4:

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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